Same Day Transduction and In Vivo Expansion of a Chimeric Antigen Receptor with a Synthetic Driver Construct for Adoptive Cellular Therapy

Frederic Vigant, Jianfang Hu, Laurence Jadin, Benjamin Lopez, Tiffany Lam, Hiba Shaban, Anirban Kundu, Timothy Mayall, Gregory Schreiber, Farzad Haerizadeh, James Onuffer, Gregory Frost

AACR 2019 Abstract #2327

Link to poster

Key Messages:


  • We demonstrate viral transfer feasibility in a point of care (POC) platform using an engineered lentiviral vector retargeted to CD3, which efficiently transduces freshly isolated resting human PBMCs in four hours
  • POC modified cells successfully engraft and expand in vivo upon IV administration in mice
  • The entire process of PBMCs isolation, genetic modification and dosing can be completed in less than twelve hours, vein to vein
  • Altogether, this platform represents a significant step forward in advancing the development of CAR-T therapies with POC potentially expanding patient accessibility and deployment